Question 22014BiologyWAEC

In a Biuret test, some protein was mixed with sodium hydroxide solution. Which of the following chemicals should be added to the mixture for a positive result?

A

Mercurous nitrate

B

Copper sulphate

C

Sodium carbonate

D

Silver nitrate

Step-by-Step Explanation

The Biuret test is used to detect the presence of proteins by forming a complex with copper ions. When sodium hydroxide is added to the protein solution, copper sulfate is then introduced, which reacts with the peptide bonds in proteins, resulting in a color change to violet. This color change indicates a positive result for protein presence.

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